Hotspot

ndhD

SSC · 122270-123790 · 1521 bp

Back to Hodgsonia

Pi 0.49

MarkerSeek score 81.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 656
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCTATTGGCCCAGCTATTGT	GCCTGACGAACACAAACATG	50.0	50.0	58.0	58.0	2458 bp	0.5	59.6
ndhD_p2	AAACAACTCGAAGCATGGGT	GCCTGACGAACACAAACATG	45.0	50.0	58.0	58.0	1909 bp	0.5	59.6
ndhD_p3	GGCATGAAACAACTCGAAGC	GCCTGACGAACACAAACATG	50.0	50.0	58.0	58.0	1915 bp	0.5	59.5
ndhD_p4	GAACGGAAGACTGTGTTGGT	GCCTGACGAACACAAACATG	50.0	50.0	58.1	58.0	1993 bp	0.5	59.3
ndhD_p5	CCGACGGATTTCTTGAGTGT	GCCTGACGAACACAAACATG	50.0	50.0	57.9	58.0	1949 bp	0.5	59.1