Hotspot

ccsA

SSC · 124042-125019 · 978 bp

Back to Hodgsonia

Pi 0.456

MarkerSeek score 81.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 382
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGTATGAGCGGTTTTGTTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1740 bp	0.5	59.8
ccsA_p2	GGTATGAGCGGTTTTGTTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1739 bp	0.5	59.7
ccsA_p3	TTATCGATGGCTTCCCTTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1767 bp	0.5	59.7
ccsA_p4	TTATCGATGGCTTCCCTTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1766 bp	0.5	59.7
ccsA_p5	CATGTTTGTGTTCGTCAGGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1189 bp	0.5	59.6