Hotspot

rpl32-ndhF

SSC · 126362-127010 · 649 bp

Back to Hodgsonia

Pi 0.415

MarkerSeek score 80.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 649
variable_sites: 225
pi: 0.415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCTTTGCTTTTCCAAAT	CCCCACGCGATTAGTTACAA	45.0	50.0	57.8	58.0	1278 bp	0.5	58.5
rpl32-ndhF_p2	AAAAGCTTTCAATGCTGCCC	CCCCACGCGATTAGTTACAA	45.0	50.0	57.8	58.0	1300 bp	0.5	58.5
rpl32-ndhF_p3	CGCCCTTTGCTTTTCCAAAT	AAACACTCGGAAAGGACCAC	45.0	50.0	57.8	58.0	859 bp	0.5	58.4
rpl32-ndhF_p4	AAAAGCTTTCAATGCTGCCC	AAACACTCGGAAAGGACCAC	45.0	50.0	57.8	58.0	881 bp	0.5	58.3
rpl32-ndhF_p5	CGCCCTTTGCTTTTCCAAAT	AGGACGGCGTACAAAGTAAC	45.0	50.0	57.8	57.9	1418 bp	0.5	58.1