Hotspot

trnL-rpl32

SSC · 125204-126199 · 996 bp

Back to Hodgsonia

Pi 0.412

MarkerSeek score 82.90

Primer available yes

Label trnL-rpl32

Feature Metadata

label_name: trnL-rpl32
feature_type: igs
region: SSC
length_bp: 996
variable_sites: 378
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-rpl32_p1	GGAGAGCGGGGACTAATAGT	AGCCTACAGGTTGATCCAGT	55.0	50.0	58.0	58.0	2026 bp	0.5	59.7
trnL-rpl32_p2	TGGAGAGCGGGGACTAATAG	AGCCTACAGGTTGATCCAGT	55.0	50.0	58.0	58.0	2027 bp	0.5	59.6
trnL-rpl32_p3	AGCGTGTCTACCGATTTCAC	AGCCTACAGGTTGATCCAGT	50.0	50.0	58.0	58.0	1471 bp	0.5	59.6
trnL-rpl32_p4	CAGCGTGTCTACCGATTTCA	AGCCTACAGGTTGATCCAGT	50.0	50.0	58.0	58.0	1472 bp	0.5	59.6
trnL-rpl32_p5	CCCTTTTCTGACGAAGCGTA	AGCCTACAGGTTGATCCAGT	50.0	50.0	57.9	58.0	1757 bp	0.5	59.1