Hotspot

ccsA-trnL

SSC · 125020-125123 · 104 bp

Back to Hodgsonia

Pi 0.406

MarkerSeek score 75.30

Primer available yes

Label ccsA-trnL

Feature Metadata

label_name: ccsA-trnL
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 41
pi: 0.406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL_p1	GGAGAGCGGGGACTAATAGT	GTGAAATCGGTAGACACGCT	55.0	50.0	58.0	58.0	575 bp	0.5	59.9
ccsA-trnL_p2	GGAGAGCGGGGACTAATAGT	TGAAATCGGTAGACACGCTG	55.0	50.0	58.0	58.0	574 bp	0.5	59.9
ccsA-trnL_p3	TGGAGAGCGGGGACTAATAG	GTGAAATCGGTAGACACGCT	55.0	50.0	58.0	58.0	576 bp	0.5	59.9
ccsA-trnL_p4	TGGAGAGCGGGGACTAATAG	TGAAATCGGTAGACACGCTG	55.0	50.0	58.0	58.0	575 bp	0.5	59.9
ccsA-trnL_p5	GGAGAGCGGGGACTAATAGT	GAGAAAGCCGCTATGGTGAA	55.0	50.0	58.0	58.0	590 bp	0.5	59.8