Hotspot

ndhD-ccsA

SSC · 123791-124041 · 251 bp

Back to Hodgsonia

Pi 0.479

MarkerSeek score 76.70

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 115
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CCTCCGTCTCTCATATGGGT	ACTATTAGTCCCCGCTCTCC	55.0	55.0	58.0	58.0	1430 bp	0.5	60.0
ndhD-ccsA_p2	CCTCCGTCTCTCATATGGGT	CTATTAGTCCCCGCTCTCCA	55.0	55.0	58.0	58.0	1429 bp	0.5	60.0
ndhD-ccsA_p3	GGTATGAGCGGTTTTGTTGC	ACTATTAGTCCCCGCTCTCC	50.0	55.0	58.0	58.0	1185 bp	0.5	59.8
ndhD-ccsA_p4	GGTATGAGCGGTTTTGTTGC	CTATTAGTCCCCGCTCTCCA	50.0	55.0	58.0	58.0	1184 bp	0.5	59.8
ndhD-ccsA_p5	TTATCGATGGCTTCCCTTGC	ACTATTAGTCCCCGCTCTCC	50.0	55.0	58.0	58.0	1212 bp	0.5	59.7