Hotspot

ndhE-psaC

SSC · 121652-121902 · 251 bp

Back to Hodgsonia

Pi 0.393

MarkerSeek score 75.70

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 88
pi: 0.393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GCTATTGGCCCAGCTATTGT	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	569 bp	0.5	59.7
ndhE-psaC_p2	GCTATTGGCCCAGCTATTGT	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	563 bp	0.5	59.6
ndhE-psaC_p3	GTTATCGCACACATTCCCCT	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	977 bp	0.5	59.5
ndhE-psaC_p4	GCTATTGGCCCAGCTATTGT	ACCAACACAGTCTTCCGTTC	50.0	50.0	58.0	58.1	485 bp	0.5	59.5
ndhE-psaC_p5	GTTATCGCACACATTCCCCT	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	971 bp	0.5	59.4