Hotspot

petG-trnW

LSC · 66880-66995 · 116 bp

Back to Hodgsonia

Pi 0.0862

MarkerSeek score 85.80

Primer available yes

Label petG-trnW

Feature Metadata

label_name: petG-trnW
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 10
pi: 0.0862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW_p1	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.2	57.9	176 bp	1.0	82.5
petG-trnW_p2	GGGGATCAGTTGGACCTTTG	CCCGATGTCGTAGGTTCAAA	55.0	50.0	58.2	57.6	171 bp	1.0	80.4
petG-trnW_p3	GGGGATCAGTTGGACCTTTG	TCCAAAACCCGATGTCGTAG	55.0	50.0	58.2	57.6	178 bp	1.0	80.2
petG-trnW_p4	GGGGATCAGTTGGACCTTTG	ACGCTCTTAGTTCAGTTCGG	55.0	50.0	58.2	57.7	211 bp	1.0	79.7
petG-trnW_p5	GGGGATCAGTTGGACCTTTG	CGCTCTTAGTTCAGTTCGGT	55.0	50.0	58.2	57.7	210 bp	1.0	79.7