primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
petG-trnW_p1	petG-trnW	1	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	20	20	55.0	50.0	58.2	57.9	0.0	1.4	0.249526	66880	66995	176-176	1.000000	82.5
petG-trnW_p2	petG-trnW	2	GGGGATCAGTTGGACCTTTG	CCCGATGTCGTAGGTTCAAA	20	20	55.0	50.0	58.2	57.6	0.0	1.4	0.534117	66880	66995	171-171	1.000000	80.4
petG-trnW_p3	petG-trnW	3	GGGGATCAGTTGGACCTTTG	TCCAAAACCCGATGTCGTAG	20	20	55.0	50.0	58.2	57.6	0.0	1.4	0.534117	66880	66995	178-178	1.000000	80.2
petG-trnW_p4	petG-trnW	4	GGGGATCAGTTGGACCTTTG	ACGCTCTTAGTTCAGTTCGG	20	20	55.0	50.0	58.2	57.7	0.0	0.0	0.509874	66880	66995	211-211	1.000000	79.7
petG-trnW_p5	petG-trnW	5	GGGGATCAGTTGGACCTTTG	CGCTCTTAGTTCAGTTCGGT	20	20	55.0	50.0	58.2	57.7	0.0	0.0	0.509874	66880	66995	210-210	1.000000	79.7
ycf1_p1	ycf1	1	ACGGTATTCCCAACAATCGG	TTTCGGGGAGAACCAAAAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042974	112202	112321	732-732	0.500000	59.7
ycf1_p2	ycf1	2	ACGGTATTCCCAACAATCGG	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055624	112202	112321	738-738	0.500000	59.6
ycf1_p3	ycf1	3	ACGGTATTCCCAACAATCGG	AAATCGTCGGACCATTCAGG	20	20	50.0	50.0	58.0	58.0	0.0	1.9	0.056856	112202	112321	474-474	0.500000	59.5
ycf1_p4	ycf1	4	ACGGTATTCCCAACAATCGG	CGTCGGACCATTCAGGAAAT	20	20	50.0	50.0	58.0	58.0	0.0	1.9	0.056856	112202	112321	470-470	0.500000	59.5
ycf1_p5	ycf1	5	ACGGTATTCCCAACAATCGG	AGCTTCCATTCCCCAAACTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.103812	112202	112321	706-706	0.500000	59.2
ndhH_p1	ndhH	1	AAGGAGGGGGAGGAGATTTC	TTGAAGAACTCCCAAAGGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.104370	116203	117384	1806-1806	0.500000	59.2
ndhH_p2	ndhH	2	CGGAAAACGCCAACGATTAC	TTGAAGAACTCCCAAAGGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.131329	116203	117384	1611-1611	0.500000	58.9
ndhH_p3	ndhH	3	AAGGAGGGGGAGGAGATTTC	GGAGCAACACTTGAAATGGC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.170007	116203	117384	2009-2009	0.500000	58.6
ndhH_p4	ndhH	4	AAGGAGGGGGAGGAGATTTC	AAAGGCCCAGCATATTCAGG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.177433	116203	117384	1793-1793	0.500000	58.6
ndhH_p5	ndhH	5	GGAGGGGGAGGAGATTTCAT	TTGAAGAACTCCCAAAGGCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.178176	116203	117384	1804-1804	0.500000	58.6
ndhA_p1	ndhA	1	ATTGGGGGTTATCAGGACCA	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.057497	117386	119638	2927-2927	0.500000	59.5
ndhA_p2	ndhA	2	ATTGGGGGTTATCAGGACCA	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.057497	117386	119638	2928-2928	0.500000	59.5
ndhA_p3	ndhA	3	TGGGGGTTATCAGGACCAAT	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.057497	117386	119638	2925-2925	0.500000	59.5
ndhA_p4	ndhA	4	TGGGGGTTATCAGGACCAAT	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.057497	117386	119638	2926-2926	0.500000	59.5
ndhA_p5	ndhA	5	TAGGGGATCAGGGTGGTTTT	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	57.9	58.1	0.0	8.8	0.120727	117386	119638	2549-2549	0.500000	59.0
ndhG_p1	ndhG	1	CTCCCCGTTGTTGATTGGAA	ACAATAGCTGGGCCAATAGC	20	20	50.0	50.0	58.1	58.0	0.0	8.0	0.108831	120586	121116	1638-1638	0.500000	59.1
ndhG_p2	ndhG	2	CTCCCCGTTGTTGATTGGAA	AGGGGAATGTGTGCGATAAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.134100	120586	121116	1230-1230	0.500000	58.9
ndhG_p3	ndhG	3	ATCTCCCCGTTGTTGATTGG	ACAATAGCTGGGCCAATAGC	20	20	50.0	50.0	57.9	58.0	0.0	8.0	0.141888	120586	121116	1640-1640	0.500000	58.9
ndhG_p4	ndhG	4	ATCTCCCCGTTGTTGATTGG	AGGGGAATGTGTGCGATAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167157	120586	121116	1232-1232	0.500000	58.7
ndhG_p5	ndhG	5	CTCCCCGTTGTTGATTGGAA	GGGGAATGTGTGCGATAACA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.350481	120586	121116	1229-1229	0.500000	57.2
ndhE_p1	ndhE	1	TTCGTTGGGACTGGTTCTTG	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056950	121349	121651	1441-1441	0.500000	59.5
ndhE_p2	ndhE	2	GTTATCGCACACATTCCCCT	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.057130	121349	121651	977-977	0.500000	59.5
ndhE_p3	ndhE	3	TTCGTTGGGACTGGTTCTTG	GCTTCGAGTTGTTTCATGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069103	121349	121651	1435-1435	0.500000	59.4
ndhE_p4	ndhE	4	GTTATCGCACACATTCCCCT	GCTTCGAGTTGTTTCATGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069284	121349	121651	971-971	0.500000	59.4
ndhE_p5	ndhE	5	TTCGTTGGGACTGGTTCTTG	ACCAACACAGTCTTCCGTTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088741	121349	121651	1357-1357	0.500000	59.3
ndhE-psaC_p1	ndhE-psaC	1	GCTATTGGCCCAGCTATTGT	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	12.5	0.0	0.031861	121652	121902	569-569	0.500000	59.7
ndhE-psaC_p2	ndhE-psaC	2	GCTATTGGCCCAGCTATTGT	GCTTCGAGTTGTTTCATGCC	20	20	50.0	50.0	58.0	58.0	12.5	0.0	0.044014	121652	121902	563-563	0.500000	59.6
ndhE-psaC_p3	ndhE-psaC	3	GTTATCGCACACATTCCCCT	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.057130	121652	121902	977-977	0.500000	59.5
ndhE-psaC_p4	ndhE-psaC	4	GCTATTGGCCCAGCTATTGT	ACCAACACAGTCTTCCGTTC	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.063652	121652	121902	485-485	0.500000	59.5
ndhE-psaC_p5	ndhE-psaC	5	GTTATCGCACACATTCCCCT	GCTTCGAGTTGTTTCATGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069284	121652	121902	971-971	0.500000	59.4
psaC_p1	psaC	1	GCTATTGGCCCAGCTATTGT	CCTCCCGCAGTGTACAAAAT	20	20	50.0	50.0	58.0	58.2	12.5	0.0	0.191901	121903	122148	1241-1241	0.500000	58.5
psaC_p2	psaC	2	GCTATTGGCCCAGCTATTGT	AAGGAGCTCGAGTACACAGA	20	20	50.0	50.0	58.0	57.8	12.5	15.1	0.215038	121903	122148	855-855	0.500000	58.3
psaC_p3	psaC	3	GCTATTGGCCCAGCTATTGT	AGAAGGAGCTCGAGTACACA	20	20	50.0	50.0	58.0	57.8	12.5	15.1	0.215038	121903	122148	857-857	0.500000	58.3
psaC_p4	psaC	4	GTTATCGCACACATTCCCCT	CCTCCCGCAGTGTACAAAAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.217170	121903	122148	1649-1649	0.500000	58.3
psaC_p5	psaC	5	GCTATTGGCCCAGCTATTGT	AACCCCGTCAGTAAAATGGG	20	20	50.0	50.0	58.0	57.8	12.5	0.0	0.222808	121903	122148	1004-1004	0.500000	58.2
ndhD_p1	ndhD	1	GCTATTGGCCCAGCTATTGT	GCCTGACGAACACAAACATG	20	20	50.0	50.0	58.0	58.0	12.5	0.0	0.049674	122270	123790	2458-2458	0.500000	59.6
ndhD_p2	ndhD	2	AAACAACTCGAAGCATGGGT	GCCTGACGAACACAAACATG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.055300	122270	123790	1909-1909	0.500000	59.6
ndhD_p3	ndhD	3	GGCATGAAACAACTCGAAGC	GCCTGACGAACACAAACATG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067453	122270	123790	1915-1915	0.500000	59.5
ndhD_p4	ndhD	4	GAACGGAAGACTGTGTTGGT	GCCTGACGAACACAAACATG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087091	122270	123790	1993-1993	0.500000	59.3
ndhD_p5	ndhD	5	CCGACGGATTTCTTGAGTGT	GCCTGACGAACACAAACATG	20	20	50.0	50.0	57.9	58.0	8.6	0.0	0.116843	122270	123790	1949-1949	0.500000	59.1
ndhD-ccsA_p1	ndhD-ccsA	1	CCTCCGTCTCTCATATGGGT	ACTATTAGTCCCCGCTCTCC	20	20	55.0	55.0	58.0	58.0	1.7	0.0	0.004283	123791	124041	1430-1430	0.500000	60.0
ndhD-ccsA_p2	ndhD-ccsA	2	CCTCCGTCTCTCATATGGGT	CTATTAGTCCCCGCTCTCCA	20	20	55.0	55.0	58.0	58.0	1.7	0.0	0.005707	123791	124041	1429-1429	0.500000	60.0
ndhD-ccsA_p3	ndhD-ccsA	3	GGTATGAGCGGTTTTGTTGC	ACTATTAGTCCCCGCTCTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.024589	123791	124041	1185-1185	0.500000	59.8
ndhD-ccsA_p4	ndhD-ccsA	4	GGTATGAGCGGTTTTGTTGC	CTATTAGTCCCCGCTCTCCA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.026013	123791	124041	1184-1184	0.500000	59.8
ndhD-ccsA_p5	ndhD-ccsA	5	TTATCGATGGCTTCCCTTGC	ACTATTAGTCCCCGCTCTCC	20	20	50.0	55.0	58.0	58.0	8.5	0.0	0.034459	123791	124041	1212-1212	0.500000	59.7
ccsA_p1	ccsA	1	GGTATGAGCGGTTTTGTTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030642	124042	125019	1740-1740	0.500000	59.8
ccsA_p2	ccsA	2	GGTATGAGCGGTTTTGTTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032012	124042	125019	1739-1739	0.500000	59.7
ccsA_p3	ccsA	3	TTATCGATGGCTTCCCTTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	8.5	0.0	0.040512	124042	125019	1767-1767	0.500000	59.7
ccsA_p4	ccsA	4	TTATCGATGGCTTCCCTTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	8.5	0.0	0.041882	124042	125019	1766-1766	0.500000	59.7
ccsA_p5	ccsA	5	CATGTTTGTGTTCGTCAGGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044387	124042	125019	1189-1189	0.500000	59.6
ccsA-trnL_p1	ccsA-trnL	1	GGAGAGCGGGGACTAATAGT	GTGAAATCGGTAGACACGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009608	125020	125123	575-575	0.500000	59.9
ccsA-trnL_p2	ccsA-trnL	2	GGAGAGCGGGGACTAATAGT	TGAAATCGGTAGACACGCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.010978	125020	125123	574-574	0.500000	59.9
ccsA-trnL_p3	ccsA-trnL	3	TGGAGAGCGGGGACTAATAG	GTGAAATCGGTAGACACGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.011032	125020	125123	576-576	0.500000	59.9
ccsA-trnL_p4	ccsA-trnL	4	TGGAGAGCGGGGACTAATAG	TGAAATCGGTAGACACGCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.012402	125020	125123	575-575	0.500000	59.9
ccsA-trnL_p5	ccsA-trnL	5	GGAGAGCGGGGACTAATAGT	GAGAAAGCCGCTATGGTGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025953	125020	125123	590-590	0.500000	59.8
trnL_p1	trnL	1	GGAGAGCGGGGACTAATAGT	CGGGATGGGGAAAATACGAA	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.337093	125124	125203	1208-1208	0.500000	57.3
trnL_p2	trnL	2	GGAGAGCGGGGACTAATAGT	TTCGGGATGGGGAAAATACG	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.337093	125124	125203	1210-1210	0.500000	57.3
trnL_p3	trnL	3	TGGAGAGCGGGGACTAATAG	CGGGATGGGGAAAATACGAA	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.338517	125124	125203	1209-1209	0.500000	57.3
trnL_p4	trnL	4	TGGAGAGCGGGGACTAATAG	TTCGGGATGGGGAAAATACG	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.338517	125124	125203	1211-1211	0.500000	57.3
trnL_p5	trnL	5	TGTTCCCCTCGTTCAGGTAT	CGGGATGGGGAAAATACGAA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.388331	125124	125203	1406-1406	0.500000	56.9
trnL-rpl32_p1	trnL-rpl32	1	GGAGAGCGGGGACTAATAGT	AGCCTACAGGTTGATCCAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.043489	125204	126199	2026-2026	0.500000	59.7
trnL-rpl32_p2	trnL-rpl32	2	TGGAGAGCGGGGACTAATAG	AGCCTACAGGTTGATCCAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.044913	125204	126199	2027-2027	0.500000	59.6
trnL-rpl32_p3	trnL-rpl32	3	AGCGTGTCTACCGATTTCAC	AGCCTACAGGTTGATCCAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049542	125204	126199	1471-1471	0.500000	59.6
trnL-rpl32_p4	trnL-rpl32	4	CAGCGTGTCTACCGATTTCA	AGCCTACAGGTTGATCCAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050913	125204	126199	1472-1472	0.500000	59.6
trnL-rpl32_p5	trnL-rpl32	5	CCCTTTTCTGACGAAGCGTA	AGCCTACAGGTTGATCCAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.116259	125204	126199	1757-1757	0.500000	59.1
rpl32-ndhF_p1	rpl32-ndhF	1	CGCCCTTTGCTTTTCCAAAT	CCCCACGCGATTAGTTACAA	20	20	45.0	50.0	57.8	58.0	0.0	15.9	0.181263	126362	127010	1278-1278	0.500000	58.5
rpl32-ndhF_p2	rpl32-ndhF	2	AAAAGCTTTCAATGCTGCCC	CCCCACGCGATTAGTTACAA	20	20	45.0	50.0	57.8	58.0	13.1	15.9	0.187300	126362	127010	1300-1300	0.500000	58.5
rpl32-ndhF_p3	rpl32-ndhF	3	CGCCCTTTGCTTTTCCAAAT	AAACACTCGGAAAGGACCAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.205154	126362	127010	859-859	0.500000	58.4
rpl32-ndhF_p4	rpl32-ndhF	4	AAAAGCTTTCAATGCTGCCC	AAACACTCGGAAAGGACCAC	20	20	45.0	50.0	57.8	58.0	13.1	0.0	0.211191	126362	127010	881-881	0.500000	58.3
rpl32-ndhF_p5	rpl32-ndhF	5	CGCCCTTTGCTTTTCCAAAT	AGGACGGCGTACAAAGTAAC	20	20	45.0	50.0	57.8	57.9	0.0	26.1	0.238588	126362	127010	1418-1418	0.500000	58.1
ndhF_p1	ndhF	1	ACTGGATCAACCTGTAGGCT	ACGAAGGGGACTAAACAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061519	127011	129260	2996-2996	0.500000	59.5
ndhF_p2	ndhF	2	ACTGGATCAACCTGTAGGCT	ACAGGAACAAGAGGGATCCA	20	20	50.0	50.0	58.0	58.0	0.0	15.2	0.086504	127011	129260	2982-2982	0.500000	59.3
ndhF_p3	ndhF	3	ACTGGATCAACCTGTAGGCT	AAGAGGGATCCACCGAAGAA	20	20	50.0	50.0	58.0	58.0	0.0	15.2	0.089090	127011	129260	2974-2974	0.500000	59.3
ndhF_p4	ndhF	4	ACTGGATCAACCTGTAGGCT	AGGGATCCACCGAAGAAGAT	20	20	50.0	50.0	58.0	57.8	0.0	15.2	0.224228	127011	129260	2971-2971	0.500000	58.2
ndhF_p5	ndhF	5	ACTGGATCAACCTGTAGGCT	CTTCTTACCCTCCGGATGGA	20	20	50.0	55.0	58.0	58.2	0.0	30.1	0.252189	127011	129260	2800-2800	0.500000	58.0