Hotspot

rps18

LSC · 72304-72609 · 306 bp

Back to Hirtella

Pi 0.00261

MarkerSeek score 25.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 2
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AACGATAGCGGCTTTACAGG	CCGGGAGAATGAATTCCGAG	50.0	55.0	58.1	58.1	818-825 bp	1.0	82.3
rps18_p2	AACGATAGCGGCTTTACAGG	ACGAGGATATATAGCGCGGA	50.0	50.0	58.1	58.0	1204-1211 bp	1.0	82.2
rps18_p3	CGGAAAATCGAAGCGTTGAG	ACGAGGATATATAGCGCGGA	50.0	50.0	57.9	58.0	1461-1468 bp	1.0	81.1
rps18_p4	AACGATAGCGGCTTTACAGG	GAAAGTGGAAGGGGTACGTC	50.0	55.0	58.1	57.9	868-870 bp	0.8	72.8
rps18_p5	CGGAAAATCGAAGCGTTGAG	GAAAGTGGAAGGGGTACGTC	50.0	55.0	57.9	57.9	1125-1127 bp	0.8	71.8