Hotspot

ndhA

SSC · 127059-129364 · 2306 bp

Back to Hirtella

Pi 0.00624

MarkerSeek score 50.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2306
variable_sites: 36
pi: 0.00624
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTGTTTGAGACAAGACAGAA	TGGGAGAAGTTGATCGTTGA	35.0	45.0	52.6	56.5	2359-2365 bp	1.0	41.5
ndhA_p2	TCTCTTGTTTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	33.3	45.0	53.5	56.5	2368-2374 bp	1.0	41.5
ndhA_p3	TCTCTTGTTTTGTTTGAGACAA	TGGGAGAAGTTGATCGTTGA	31.8	45.0	54.4	56.5	2368-2374 bp	1.0	41.5
ndhA_p4	TGTTTGAGACAAGACAGAAT	TGGGAGAAGTTGATCGTTGA	35.0	45.0	52.3	56.5	2358-2364 bp	1.0	41.5
ndhA_p5	TTCTCTTGTTTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	31.8	45.0	54.4	56.5	2369-2375 bp	1.0	41.4