Hotspot

ndhF-rpl32

SSC · 118523-119647 · 1125 bp

Back to Hirtella

Pi 0.0149

MarkerSeek score 65.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1125
variable_sites: 39
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAAGTGTAACGAAGGGT	TTTTTCCGATGTCAAGCCGA	50.0	45.0	58.1	58.1	1285-1406 bp	1.0	84.7
ndhF-rpl32_p2	CGATGCAATAGGTCGTGTGA	TTTTTCCGATGTCAAGCCGA	50.0	45.0	58.1	58.1	1749-1870 bp	1.0	84.6
ndhF-rpl32_p3	TCGATGCAATAGGTCGTGTG	TTTTTCCGATGTCAAGCCGA	50.0	45.0	58.1	58.1	1750-1871 bp	1.0	84.6
ndhF-rpl32_p4	ATGGGTCAATCAAGTGTCCG	TTTTTCCGATGTCAAGCCGA	50.0	45.0	57.9	58.1	1507-1628 bp	1.0	84.6
ndhF-rpl32_p5	CGATGCAATAGGTCGTGTGA	AAAAAGCTTTCAACGCGGTC	50.0	45.0	58.1	58.2	1646-1767 bp	1.0	84.2