Hotspot

rpl20-rps12

LSC · 73254-74073 · 820 bp

Back to Hirtella

Pi 0.00341
MarkerSeek score 56.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
820
variable_sites
7
pi
0.00341
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGCGCTATATATCCTCGT GAGGAACATGTACTCGGGTG 50.0 55.0 58.0 57.7 876-892 bp 1.0 78.7
rpl20-rps12_p2 TCCGCGCTATATATCCTCGT AGGAACATGTACTCGGGTGT 50.0 50.0 58.0 58.4 875-891 bp 1.0 78.2
rpl20-rps12_p3 TCCGCGCTATATATCCTCGT AAATGTCACCAAATCCCCCG 50.0 50.0 58.0 58.4 920-936 bp 1.0 77.5
rpl20-rps12_p4 AGTAAGTCTTGAATGCGCCC GAGGAACATGTACTCGGGTG 50.0 55.0 58.3 57.7 938-954 bp 1.0 76.8
rpl20-rps12_p5 AGTAAGTCTTGAATGCGCCC AGGAACATGTACTCGGGTGT 50.0 50.0 58.3 58.4 937-953 bp 1.0 76.3