Hotspot

rpl33-rps18

LSC · 72026-72303 · 278 bp

Back to Hirtella

Pi 0.0302

MarkerSeek score 70.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 17
pi: 0.0302
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGACACAATACGCCT	GGGGTCGCTTGGACTTAAAA	45.0	50.0	57.0	58.1	407-413 bp	1.0	77.7
rpl33-rps18_p2	CTGTCCCTATTGTTCCAAGCA	GGGGTCGCTTGGACTTAAAA	47.6	50.0	57.9	58.1	364-370 bp	1.0	77.6
rpl33-rps18_p3	CGACACAATACGCCTAATCG	GGGGTCGCTTGGACTTAAAA	50.0	50.0	56.8	58.1	402-408 bp	1.0	76.0
rpl33-rps18_p4	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	450-456 bp	1.0	75.9
rpl33-rps18_p5	CTGTCCCTATTGTTCCAAGCA	TCAATTCGATCCCCCGATTG	47.6	50.0	57.9	57.7	407-413 bp	1.0	75.6