Hotspot

ndhC-trnV-UAC

LSC · 53172-54322 · 1151 bp

Back to Hirtella

Pi 0.0155
MarkerSeek score 67.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1151
variable_sites
39
pi
0.0155
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATGGGGGATAAAACTCCGGA GCATGTTGGGTCTTTGAAGC 50.0 50.0 58.1 57.9 1328-1367 bp 1.0 83.4
ndhC-trnV-UAC_p2 TCCGGAAATTCGAAATGCCA GCATGTTGGGTCTTTGAAGC 45.0 50.0 58.2 57.9 1314-1353 bp 1.0 82.9
ndhC-trnV-UAC_p3 ATGGGGGATAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1264-1303 bp 1.0 82.6
ndhC-trnV-UAC_p4 TCCGGAAATTCGAAATGCCA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.2 57.8 1250-1289 bp 1.0 82.1
ndhC-trnV-UAC_p5 TGGGGGATAAAACTCCGGAA GCATGTTGGGTCTTTGAAGC 50.0 50.0 58.3 57.9 1327-1366 bp 1.0 81.6