Hotspot

trnS-GGA-rps4

LSC · 47827-48122 · 296 bp

Back to Hirtella

Pi 0.0172
MarkerSeek score 57.60
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
296
variable_sites
12
pi
0.0172
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 TTCGAATCCCTCTCTTTCCG TCCAACACAAAGGATTGGTCA 50.0 42.9 57.0 57.7 399-417 bp 1.0 68.2
trnS-GGA-rps4_p2 GGGTTCGAATCCCTCTCTTT TCCAACACAAAGGATTGGTCA 50.0 42.9 56.9 57.7 402-420 bp 1.0 67.2
trnS-GGA-rps4_p3 GGGTTCGAATCCCTCTCTTTC TCCAACACAAAGGATTGGTCA 52.4 42.9 58.1 57.7 402-420 bp 1.0 67.1
trnS-GGA-rps4_p4 GGTTCGAATCCCTCTCTTTCC TCCAACACAAAGGATTGGTCA 52.4 42.9 58.1 57.7 401-419 bp 1.0 67.1
trnS-GGA-rps4_p5 GTTCGAATCCCTCTCTTTCCG TCCAACACAAAGGATTGGTCA 52.4 42.9 58.5 57.7 400-418 bp 1.0 64.2