Hotspot

trnE-UUC-trnT-GGU

LSC · 32830-33509 · 680 bp

Back to Hirtella

Pi 0.0179

MarkerSeek score 59.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 30
pi: 0.0179
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CCATTTCGTGGTGGATCAGT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1037-1076 bp	1.0	84.4
trnE-UUC-trnT-GGU_p2	CCATTTCGTGGTGGATCAGT	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.9	57.9	1002-1041 bp	1.0	83.8
trnE-UUC-trnT-GGU_p3	TGCCACGTTGTAACAGAGAC	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1389-1428 bp	1.0	83.0
trnE-UUC-trnT-GGU_p4	CCATTTCGTGGTGGATCAGT	ACTTTTCTGCGGAAGAAGGG	50.0	50.0	57.9	58.1	1596-1641 bp	1.0	82.5
trnE-UUC-trnT-GGU_p5	TGCCACGTTGTAACAGAGAC	ACTTTTCTGCGGAAGAAGGG	50.0	50.0	58.1	58.1	1948-1993 bp	1.0	81.6