Hotspot

petN-psbM

LSC · 29799-30903 · 1105 bp

Back to Hirtella

Pi 0.00489

MarkerSeek score 57.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 13
pi: 0.00489
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	58.2	58.0	1217-1260 bp	1.0	82.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	57.6	58.0	1156-1199 bp	1.0	81.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1226-1269 bp	1.0	80.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1227-1270 bp	1.0	79.7
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACTGCGCTGTTCATTCTTGT	50.0	45.0	58.2	58.4	1220-1263 bp	1.0	79.5