Hotspot

trnG-GCC-trnR-UCU

LSC · 8992-9646 · 655 bp

Back to Hirtella

Pi 0.00733

MarkerSeek score 65.10

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 12
pi: 0.00733
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	735-811 bp	1.0	68.6
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	738-814 bp	1.0	68.4
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	741-817 bp	1.0	67.9
trnG-GCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	744-820 bp	1.0	67.7
trnG-GCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	739-815 bp	1.0	66.2