Hotspot

trnV-UAC

LSC · 51099-51135 · 37 bp

Back to Hippuris

Pi 0.162
MarkerSeek score 72.50
Primer available yes
Label trnV-UAC

Feature Metadata

label_name
trnV-UAC
feature_type
tRNA
region
LSC
length_bp
37
variable_sites
6
pi
0.162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC_p1 TTTTCAACAGGCTCGTGTCA TGAATCAGAGCACATGGAGC 45.0 50.0 58.0 58.0 550-590 bp 1.0 90.4
trnV-UAC_p2 TTTGTTGTGCTTCGCTAGGT TGAATCAGAGCACATGGAGC 45.0 50.0 58.0 58.0 713-753 bp 1.0 88.8
trnV-UAC_p3 TTTTCAACAGGCTCGTGTCA GACAAAAGGTTCGGTCCAGT 45.0 50.0 58.0 58.0 855-894 bp 1.0 88.2
trnV-UAC_p4 TTTTCAACAGGCTCGTGTCA CTGACAAAAGGTTCGGTCCA 45.0 50.0 58.0 58.0 857-896 bp 1.0 88.2
trnV-UAC_p5 TTTTCAACAGGCTCGTGTCA TGACAAAAGGTTCGGTCCAG 45.0 50.0 58.0 58.0 856-895 bp 1.0 88.2
trnV-UAC_p1 TTTTCAACAGGCTCGTGTCA AAACCAAATGGGACCCATCC 45.0 50.0 58.0 58.0 1134-1167 bp 1.0 87.3
trnV-UAC_p2 TTTTCAACAGGCTCGTGTCA CCCCTATTGCTACAGCTGTG 45.0 55.0 58.0 58.0 1704-1739 bp 1.0 86.0
trnV-UAC_p3 TTTTCAACAGGCTCGTGTCA CCCTATTGCTACAGCTGTGG 45.0 55.0 58.0 58.0 1703-1738 bp 1.0 86.0
trnV-UAC_p4 TTTGTTGTGCTTCGCTAGGT CCCCTATTGCTACAGCTGTG 45.0 55.0 58.0 58.0 1867-1902 bp 1.0 85.4
trnV-UAC_p5 TTTGTTGTGCTTCGCTAGGT CCCTATTGCTACAGCTGTGG 45.0 55.0 58.0 58.0 1866-1901 bp 1.0 85.4