Hotspot

ndhA

SSC · 117890-120075 · 2186 bp

Back to Hippuris

Pi 0.0299

MarkerSeek score 61.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2186
variable_sites: 65
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCAAAGTGTATTCGGCCTCG	TGGGAGGTTCAATGGCAAAA	50.0	45.0	58.0	57.9	2891 bp	1.0	81.5
ndhA_p2	TCAAAGTGTATTCGGCCTCG	TTGGGAGGTTCAATGGCAAA	50.0	45.0	58.0	57.9	2892 bp	1.0	81.5
ndhA_p3	TGACCAATGTATCTGGCAGC	TGGGAGGTTCAATGGCAAAA	50.0	45.0	58.0	57.9	2783 bp	1.0	81.3
ndhA_p4	TGACCAATGTATCTGGCAGC	TTGGGAGGTTCAATGGCAAA	50.0	45.0	58.0	57.9	2784 bp	1.0	81.3
ndhA_p5	CAATGTATCTGGCAGCTCGT	TGGGAGGTTCAATGGCAAAA	50.0	45.0	58.1	57.9	2779 bp	1.0	81.2