Hotspot

cemA

LSC · 60598-61287 · 690 bp

Back to Hippuris

Pi 0.0145

MarkerSeek score 54.90

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GACGAATGAGGTGGGGTAAT	ACTATTGTATTGGTGGATCATT	50.0	31.8	57.0	52.9	754 bp	1.0	41.1
cemA_p2	GACGAATGAGGTGGGGTAAT	ACTATTGTATTGGTGGATCATTT	50.0	30.4	57.0	53.8	754 bp	1.0	41.1
cemA_p3	AGAGTCGACGAATGAGGTGG	ACTATTGTATTGGTGGATCATT	55.0	31.8	59.2	52.9	760 bp	1.0	41.1
cemA_p4	GACGAATGAGGTGGGGTAATT	ACTATTGTATTGGTGGATCATT	47.6	31.8	57.7	52.9	754 bp	1.0	41.1
cemA_p5	GACGAATGAGGTGGGGTAAT	AGATTACTATTGTATTGGTGGATCA	50.0	32.0	57.0	55.6	759 bp	1.0	41.1