Hotspot

rps19-rpl2

IRb · 82730-82783 · 54 bp

Back to Hippuris

Pi 0.0926

MarkerSeek score 72.40

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 54
variable_sites: 5
pi: 0.0926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AATGGTAGATGCACGTGACC	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	399-404 bp	1.0	80.4
rps19-rpl2_p2	AGGTAGTCCCCTGGTTAACC	AAAAGTTTGGGTAGAGCCGG	55.0	50.0	58.0	58.1	915-920 bp	1.0	80.2
rps19-rpl2_p3	GGTAGTCCCCTGGTTAACCT	AAAAGTTTGGGTAGAGCCGG	55.0	50.0	58.0	58.1	914-919 bp	1.0	80.2
rps19-rpl2_p4	AATGGTAGATGCACGTGACC	GCGAACCAGAAAAGTTTGGG	50.0	50.0	58.0	57.9	408-413 bp	1.0	80.1
rps19-rpl2_p5	AATGGTAGATGCACGTGACC	GGCGAACCAGAAAAGTTTGG	50.0	50.0	58.0	57.9	409-414 bp	1.0	80.1