Hotspot

rps3-rpl22

LSC · 81941-81965 · 25 bp

Back to Hippuris

Pi 0.04
MarkerSeek score 74.60
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
25
variable_sites
1
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 GATGGCTTTGGGTTGTACCA TGAACAGCCACCTCTTTTCT 50.0 45.0 58.1 57.0 108-109 bp 1.0 71.8
rps3-rpl22_p2 TGATGGCTTTGGGTTGTACC TGAACAGCCACCTCTTTTCT 50.0 45.0 58.1 57.0 109-110 bp 1.0 71.8
rps3-rpl22_p3 AAGGGAATGATGGCTTTGGG TGAACAGCCACCTCTTTTCT 50.0 45.0 58.1 57.0 116-117 bp 1.0 71.4
rps3-rpl22_p4 GATGGCTTTGGGTTGTACCA TGAACAGCCACCTCTTTTCTT 50.0 42.9 58.1 57.7 108-109 bp 1.0 69.7
rps3-rpl22_p5 TGATGGCTTTGGGTTGTACC TGAACAGCCACCTCTTTTCTT 50.0 42.9 58.1 57.7 109-110 bp 1.0 69.7