Hotspot

cemA-petA

LSC · 61288-61501 · 214 bp

Back to Hippuris

Pi 0.103
MarkerSeek score 76.10
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
214
variable_sites
22
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 TCTCCGTCACTTGTAGTTAT GCACTTGAAATGGATGTCCG 40.0 50.0 53.1 57.5 359-360 bp 1.0 45.1
cemA-petA_p2 TCTCCGTCACTTGTAGTTATT GCACTTGAAATGGATGTCCG 38.1 50.0 54.1 57.5 359-360 bp 1.0 45.1
cemA-petA_p3 TCTCCGTCACTTGTAGTTAT CGCAAAAATGGGATAGGCAC 40.0 50.0 53.1 57.8 375-376 bp 1.0 44.9
cemA-petA_p4 TCTCCGTCACTTGTAGTTATT CGCAAAAATGGGATAGGCAC 38.1 50.0 54.1 57.8 375-376 bp 1.0 44.9
cemA-petA_p5 TCTCCGTCACTTGTAGTTATTT CGCAAAAATGGGATAGGCAC 36.4 50.0 54.9 57.8 375-376 bp 1.0 44.9