Hotspot

ycf4-cemA

LSC · 60123-60597 · 475 bp

Back to Hippuris

Pi 0.0316

MarkerSeek score 74.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 15
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	649-650 bp	1.0	80.9
ycf4-cemA_p2	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	648-649 bp	1.0	80.9
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	575-576 bp	1.0	80.7
ycf4-cemA_p4	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	604-605 bp	1.0	79.7
ycf4-cemA_p5	GCTATTCCCTTGACCCGTAC	GAGAAATCCACCAGGGCAAA	55.0	50.0	57.8	58.1	652-653 bp	1.0	79.5