Hotspot

trnK-UUU-rps16

LSC · 4180-5080 · 901 bp

Back to Hippuris

Pi 0.0307

MarkerSeek score 68.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 26
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	1018-1038 bp	1.0	84.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	1017-1037 bp	1.0	84.6
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.5	58.0	1004-1024 bp	1.0	82.3
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.2	58.0	1026-1046 bp	1.0	79.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	ATTTCCTTGAAAGGGGTGCT	50.0	45.0	58.2	57.3	1018-1038 bp	1.0	79.1