Hotspot

trnR-ACG-rrn5

IRa · 131939-132190 · 252 bp

Back to Hippophae

Pi 0.0522

MarkerSeek score 81.70

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 252
variable_sites: 34
pi: 0.0522
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CCAAAGGGAAAGGTCCTTCC	GGTGACGATACTGTAGGGGA	55.0	55.0	58.1	57.9	408-420 bp	1.0	86.6
trnR-ACG-rrn5_p2	CCAAAGGGAAAGGTCCTTCC	AATCCATCCCGAACTTGGTG	55.0	50.0	58.1	57.9	443-455 bp	1.0	85.5
trnR-ACG-rrn5_p3	CCAAAGGGAAAGGTCCTTCC	ACCAATCCATCCCGAACTTG	55.0	50.0	58.1	57.9	446-458 bp	1.0	85.5
trnR-ACG-rrn5_p4	CCAAAGGGAAAGGTCCTTCC	CAATCCATCCCGAACTTGGT	55.0	50.0	58.1	57.9	444-456 bp	1.0	85.5
trnR-ACG-rrn5_p5	CCAAAGGGAAAGGTCCTTCC	CACCAATCCATCCCGAACTT	55.0	50.0	58.1	57.9	447-459 bp	1.0	85.4