Hotspot

rps4-trnT-UGU

LSC · 45996-46670 · 675 bp

Back to Hippophae

Pi 0.023

MarkerSeek score 71.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 68
pi: 0.023
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	715-806 bp	1.0	87.9
rps4-trnT-UGU_p2	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	683-774 bp	1.0	87.3
rps4-trnT-UGU_p3	GAAACGAGGCCCTCTGTAAC	TGTTACACAAGCCCGCTTAG	55.0	50.0	58.3	58.2	735-826 bp	1.0	87.2
rps4-trnT-UGU_p4	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	720-811 bp	1.0	87.2
rps4-trnT-UGU_p5	TTTTGAAACGAGGCCCTCTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	719-810 bp	1.0	86.9