Hotspot

trnE-UUC-trnT-GGU

LSC · 30681-31358 · 678 bp

Back to Hippophae

Pi 0.0182

MarkerSeek score 70.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 46
pi: 0.0182
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	760-843 bp	1.0	84.6
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	758-841 bp	1.0	84.6
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	739-822 bp	1.0	84.3
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	737-820 bp	1.0	84.3
trnE-UUC-trnT-GGU_p5	AACTTCCTTTCGTAGCACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	821-904 bp	1.0	84.0