Hotspot

tRNA-GUC-trnY-GUA

LSC · 30146-30465 · 320 bp

Back to Hippophae

Pi 0.0254

MarkerSeek score 69.90

Primer available yes

Label tRNA-GUC-trnY-GUA

Feature Metadata

label_name: tRNA-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 30
pi: 0.0254
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	375-393 bp	1.0	84.4
tRNA-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	374-392 bp	1.0	84.4
tRNA-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	396-414 bp	1.0	83.0
tRNA-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	395-413 bp	1.0	82.8
tRNA-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	429-447 bp	1.0	81.9