Hotspot

petN-psbM

LSC · 28920-29469 · 550 bp

Back to Hippophae

Pi 0.0227

MarkerSeek score 71.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 60
pi: 0.0227
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AAAAGCATCCCCCACGAATT	CAAAGAAGGCGGTAGGTTGT	45.0	50.0	58.1	58.1	1243-1270 bp	0.846154	78.1
petN-psbM_p2	AAAGCATCCCCCACGAATTT	ACAAAGAAGGCGGTAGGTTG	45.0	50.0	58.1	58.1	1243-1270 bp	0.846154	78.1
petN-psbM_p3	AAAGCATCCCCCACGAATTT	CAAAGAAGGCGGTAGGTTGT	45.0	50.0	58.1	58.1	1242-1269 bp	0.846154	78.1
petN-psbM_p4	AAGCATCCCCCACGAATTTT	ACAAAGAAGGCGGTAGGTTG	45.0	50.0	58.1	58.1	1242-1269 bp	0.769231	74.9
petN-psbM_p5	AAGCATCCCCCACGAATTTT	CAAAGAAGGCGGTAGGTTGT	45.0	50.0	58.1	58.1	1241-1268 bp	0.769231	74.9