Hotspot

trnS-GCU-trnG-GCC

LSC · 7961-8676 · 716 bp

Back to Hippophae

Pi 0.0167

MarkerSeek score 70.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 47
pi: 0.0167
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ATCAAACCGAGGACCCCTTA	50.0	50.0	58.0	58.0	664-825 bp	1.0	88.8
trnS-GCU-trnG-GCC_p2	CAATCCGACGCTTTAGTCCA	TAATCAAACCGAGGACCCCT	50.0	50.0	58.0	58.0	666-827 bp	1.0	88.8
trnS-GCU-trnG-GCC_p3	AATCCGACGCTTTAGTCCAC	ATCAAACCGAGGACCCCTTA	50.0	50.0	58.0	58.0	663-824 bp	1.0	88.8
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	TAATCAAACCGAGGACCCCT	50.0	50.0	58.0	58.0	665-826 bp	1.0	88.8
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	CAAACCGAGGACCCCTTAAC	50.0	55.0	58.0	58.2	662-823 bp	1.0	87.7