Hotspot

rps16-trnQ-UUG

LSC · 5187-6625 · 1439 bp

Back to Hippophae

Pi 0.0172

MarkerSeek score 69.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1439
variable_sites: 109
pi: 0.0172
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCGAACATCAATTGCAACGA	GAGGTTCGAATCCTTCCGTC	40.0	55.0	56.4	58.1	1366-1549 bp	1.0	76.3
rps16-trnQ-UUG_p2	TCGAACATCAATTGCAACGA	TAAGGCATCGGGTTTTGGTC	40.0	50.0	56.4	58.2	1396-1579 bp	1.0	75.4
rps16-trnQ-UUG_p3	GATCGAACATCAATTGCAACGA	GAGGTTCGAATCCTTCCGTC	40.9	55.0	58.0	58.1	1368-1551 bp	1.0	73.3
rps16-trnQ-UUG_p4	TCGAACATCAATTGCAACGA	GTAAGGCATCGGGTTTTGGT	40.0	50.0	56.4	58.5	1397-1580 bp	1.0	73.1
rps16-trnQ-UUG_p5	TCGAACATCAATTGCAACGA	GTGGTAAGGCATCGGGTTTT	40.0	50.0	56.4	58.5	1400-1583 bp	1.0	73.1