Hotspot

rpl16

LSC · 78603-80048 · 1446 bp

Back to Hippocrepis

Pi 0.0283

MarkerSeek score 64.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 60
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCTCCGCTATTATCGGCTAC	ATGGATCCGAGAAGGTAGGG	55.0	55.0	58.1	58.0	1863-1960 bp	1.0	83.3
rpl16_p2	GTACCGTCGATTACTGGCTC	ATGGATCCGAGAAGGTAGGG	55.0	55.0	57.9	58.0	1912-2009 bp	1.0	83.0
rpl16_p3	GCTCCGCTATTATCGGCTAC	ATTGCACGCGTAGAATGGAT	55.0	45.0	58.1	58.0	1877-1974 bp	1.0	83.0
rpl16_p4	GCTCCGCTATTATCGGCTAC	GCAGAATTCATAGCCGGACA	55.0	50.0	58.1	58.0	2012-2109 bp	1.0	82.8
rpl16_p5	GCTCCGCTATTATCGGCTAC	TGCAGAATTCATAGCCGGAC	55.0	50.0	58.1	58.0	2013-2110 bp	1.0	82.8