Hotspot

rps15-ycf1

SSC · 119652-120049 · 398 bp

Back to Hippocrepis

Pi 0.0419

MarkerSeek score 74.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 22
pi: 0.0419
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTTTTCTTGTGAAATAACCGAT	ATCCACGGTTGAAAATGCGA	27.3	45.0	52.6	58.5	417-465 bp	1.0	47.9
rps15-ycf1_p2	TTTTTCTTGTGAAATAACCGAT	TCCACGGTTGAAAATGCGAT	27.3	45.0	52.6	58.5	416-464 bp	1.0	47.9
rps15-ycf1_p3	TTTTTCTTGTGAAATAACCGAT	TCCACGGTTGAAAATGCGA	27.3	47.4	52.6	58.0	416-464 bp	1.0	47.9
rps15-ycf1_p4	TTTTTCTTGTGAAATAACCGAT	ACCCATAATGGAAGCCGTTT	27.3	45.0	52.6	57.5	460-508 bp	1.0	47.6
rps15-ycf1_p5	TTTTTCTTGTGAAATAACCGAT	CCCATAATGGAAGCCGTTTC	27.3	50.0	52.6	56.8	459-507 bp	1.0	47.6