Hotspot

ndhD-psaC

SSC · 113016-113149 · 134 bp

Back to Hippocrepis

Pi 0.0498

MarkerSeek score 75.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 134
variable_sites: 8
pi: 0.0498
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAATTAAGGAACCGCCAGCT	ACTCGAAGTATGGGTCTAGCT	45.0	47.6	57.8	57.7	222-225 bp	1.0	72.2
ndhD-psaC_p2	AAATTAAGGAACCGCCAGCT	TGGCATGAAACAACTCGAAG	45.0	45.0	57.8	56.7	234-237 bp	1.0	71.5
ndhD-psaC_p3	AAATTAAGGAACCGCCAGCT	GGCATGAAACAACTCGAAGT	45.0	45.0	57.8	56.6	233-236 bp	1.0	71.5
ndhD-psaC_p4	TAAGGAACCGCCAGCTATTG	ACTCGAAGTATGGGTCTAGCT	50.0	47.6	57.7	57.7	218-221 bp	1.0	71.4
ndhD-psaC_p5	AAATTAAGGAACCGCCAGCT	TGGCATGAAACAACTCGAAGT	45.0	42.9	57.8	58.4	234-237 bp	1.0	70.9