Hotspot

rpl32-trnL-UAG

SSC · 109783-110097 · 315 bp

Back to Hippocrepis

Pi 0.0748
MarkerSeek score 80.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
315
variable_sites
31
pi
0.0748
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAATCCATTTTTACGGGAA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 54.5 57.9 369-403 bp 1.0 61.6
rpl32-trnL-UAG_p2 GCAATCCATTTTTACGGGAA AGCAGCGTGTCTACCAATTT 40.0 45.0 54.5 57.8 362-396 bp 1.0 61.3
rpl32-trnL-UAG_p3 GCAATCCATTTTTACGGGAA CCTAAGAGCAGCGTGTCTAC 40.0 55.0 54.5 57.8 368-402 bp 1.0 61.1
rpl32-trnL-UAG_p4 GCAATCCATTTTTACGGGAA CTAAGAGCAGCGTGTCTACC 40.0 55.0 54.5 57.8 367-401 bp 1.0 61.1
rpl32-trnL-UAG_p5 GCAATCCATTTTTACGGGAA GAGCAGCGTGTCTACCAATT 40.0 50.0 54.5 58.3 363-397 bp 1.0 60.6