Hotspot

ycf1-ndhF

SSC · 106634-106662 · 29 bp

Back to Hippocrepis

Pi 0.111

MarkerSeek score 76.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 29
variable_sites: 4
pi: 0.111
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ATGGAAGAAGGGACCGAGAA	TGTGGGAAGTGGACGAATTT	50.0	45.0	58.0	57.4	473-478 bp	1.0	76.9
ycf1-ndhF_p2	CGTTATGGAAGAAGGGACCG	TGTGGGAAGTGGACGAATTT	55.0	45.0	58.1	57.4	477-482 bp	1.0	76.8
ycf1-ndhF_p3	AGGGCCCTCTTATCTCTTCC	TGTGGGAAGTGGACGAATTT	55.0	45.0	57.9	57.4	510-515 bp	1.0	76.3
ycf1-ndhF_p4	GGGCCCTCTTATCTCTTCCT	TGTGGGAAGTGGACGAATTT	55.0	45.0	57.9	57.4	509-514 bp	1.0	76.3
ycf1-ndhF_p5	CACAGGGCCCTCTTATCTCT	TGTGGGAAGTGGACGAATTT	55.0	45.0	58.2	57.4	513-518 bp	1.0	75.5