Hotspot

petA-psbJ

LSC · 60479-61329 · 851 bp

Back to Hippocrepis

Pi 0.0223

MarkerSeek score 72.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 28
pi: 0.0223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.8	58.1	1023-1037 bp	1.0	81.5
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	GCTGGTATTCCTGTGATCGG	55.0	55.0	58.3	58.1	1028-1042 bp	1.0	80.9
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	GCTGGTATTCCTGTGATCGG	55.0	55.0	58.4	58.1	1032-1046 bp	1.0	80.5
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.9	58.1	1024-1038 bp	1.0	76.4
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGATCATCC	50.0	50.0	57.8	57.1	974-988 bp	1.0	75.6