Hotspot

petN-trnC-GCA

LSC · 32084-32635 · 552 bp

Back to Hippocrepis

Pi 0.0368

MarkerSeek score 75.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 30
pi: 0.0368
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAGGCAAGACTTACTA	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.0	58.1	608-1207 bp	1.0	82.7
petN-trnC-GCA_p2	AGCCCAGGCAAGACTTACTA	TTTATCGTTTTGGCGGCATG	50.0	45.0	58.0	57.7	661-1260 bp	1.0	81.1
petN-trnC-GCA_p3	AGCCCAGGCAAGACTTACTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.0	58.4	626-1225 bp	1.0	80.4
petN-trnC-GCA_p4	TCTTCCCCATACTACGAGGG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	57.6	58.1	659-1258 bp	1.0	79.9
petN-trnC-GCA_p5	TACCATTAAAGCAGCCCAGG	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	620-1219 bp	1.0	79.5