Hotspot

trnG-GCC-psbZ

LSC · 24025-24614 · 590 bp

Back to Hippocrepis

Pi 0.0383

MarkerSeek score 74.90

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 30
pi: 0.0383
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	TGCATTAGTATTTCTAGTGGGT	50.0	36.4	57.8	54.6	602-699 bp	1.0	47.6
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	GGATTGCATTAGTATTTCTAGTGGG	50.0	40.0	57.8	57.6	606-703 bp	1.0	47.6
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TTGCATTAGTATTTCTAGTGGGT	50.0	34.8	57.8	55.4	603-700 bp	1.0	47.6
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	GGTACATCATTATGGATTGCA	50.0	38.1	57.8	53.7	619-716 bp	1.0	47.5
trnG-GCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	GGTACATCATTATGGATTGCAT	50.0	36.4	57.8	54.3	619-716 bp	1.0	47.5