Hotspot

trnT-UGU-rps4

LSC · 14729-15033 · 305 bp

Back to Hippocrepis

Pi 0.0328

MarkerSeek score 65.30

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 15
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	CTCAGAGGTTAGAGCATCGC	TCTTTTTGAAACGAGGCCCT	55.0	45.0	57.9	57.6	402-428 bp	1.0	80.9
trnT-UGU-rps4_p2	ATGGTCATCGGTTCGATTCC	TCTTTTTGAAACGAGGCCCT	50.0	45.0	57.8	57.6	370-396 bp	1.0	80.5
trnT-UGU-rps4_p3	CTGTTATGTGAGCCCGCTTA	TCTTTTTGAAACGAGGCCCT	50.0	45.0	57.7	57.6	423-449 bp	1.0	79.3
trnT-UGU-rps4_p4	TGTTATGTGAGCCCGCTTAG	TCTTTTTGAAACGAGGCCCT	50.0	45.0	57.7	57.6	422-448 bp	1.0	79.3
trnT-UGU-rps4_p5	CTCAGAGGTTAGAGCATCGC	CTTTTTGAAACGAGGCCCTC	55.0	50.0	57.9	57.3	401-427 bp	1.0	78.1