Pi
0.0411
MarkerSeek score
80.80
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 762-889 bp | 1.0 | 86.0 |
| trnL-UAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 770-897 bp | 1.0 | 85.4 |
| trnL-UAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 794-921 bp | 1.0 | 84.9 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 802-929 bp | 1.0 | 84.3 |
| trnL-UAA-trnT-UGU_p5 | AGTCCGTAGCGTCTACCAAT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.2 | 57.9 | 776-903 bp | 1.0 | 84.0 |