Hotspot

ycf1

SSC · 126788-132229 · 5442 bp

Back to Hippeastrum

Pi 0.0037

MarkerSeek score 44.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5442
variable_sites: 73
pi: 0.0037
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCACTCGGGGAATTGCAATA	AGGGGAAAGTGAACGAATAGC	50.0	47.6	57.9	58.0	175 bp	1.0	75.6
ycf1_p2	CAAATCCTCGTACCACTCGG	AGGGGAAAGTGAACGAATAGC	55.0	47.6	58.1	58.0	187 bp	1.0	75.1
ycf1_p3	CCACTCGGGGAATTGCAATA	GGGGAAAGTGAACGAATAGCA	50.0	47.6	57.9	58.3	174 bp	1.0	73.4
ycf1_p4	CCACTCGGGGAATTGCAATA	AAGAAGGGGAAAGTGAACGA	50.0	45.0	57.9	56.7	179 bp	1.0	73.1
ycf1_p5	CCACTCGGGGAATTGCAATA	AGAAGGGGAAAGTGAACGAA	50.0	45.0	57.9	56.7	178 bp	1.0	73.1