Hotspot

rpoB

LSC · 23969-27181 · 3213 bp

Back to Hippeastrum

Pi 0.00112

MarkerSeek score 47.50

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 14
pi: 0.00112
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	CCGCGGGGACATATAATTCA	TCCGGAATGGAAATGAGGGA	50.0	50.0	57.5	58.4	249 bp	1.0	73.6
rpoB_p2	CCGCGGGGACATATAATTCA	CCGGAATGGAAATGAGGGAA	50.0	50.0	57.5	57.6	248 bp	1.0	73.4
rpoB_p3	TTCCAAATTAATCCCGCGGG	TCCGGAATGGAAATGAGGGA	50.0	50.0	58.6	58.4	262 bp	1.0	72.5
rpoB_p4	TTCCAAATTAATCCCGCGGG	CCGGAATGGAAATGAGGGAA	50.0	50.0	58.6	57.6	261 bp	1.0	72.3
rpoB_p5	TCCCGCGGGGACATATAATT	TCCGGAATGGAAATGAGGGA	50.0	50.0	58.6	58.4	251 bp	1.0	72.2