Hotspot

rpl2

IRa · 155988-157478 · 1491 bp

Back to Hippeastrum

Pi 0.000782
MarkerSeek score 46.90
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1491
variable_sites
4
pi
0.000782
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 CGGATGTAGCCAAGTGGATC CGCTTCAACCGGGTTATTCT 55.0 50.0 58.1 58.3 1679 bp 1.0 77.0
rpl2_p2 TGGATCAAGGCAGTGGATTG CGCTTCAACCGGGTTATTCT 50.0 50.0 57.9 58.3 1665 bp 1.0 77.0
rpl2_p3 GGATCAAGGCAGTGGATTGT CGCTTCAACCGGGTTATTCT 50.0 50.0 57.9 58.3 1664 bp 1.0 77.0
rpl2_p4 GTGGATCAAGGCAGTGGATT CGCTTCAACCGGGTTATTCT 50.0 50.0 57.9 58.3 1666 bp 1.0 77.0
rpl2_p5 CGGATGTAGCCAAGTGGATC ATCATTACGCTTCAACCGGG 55.0 50.0 58.1 58.3 1686 bp 1.0 76.5
rpl2_p1 CGCTTCAACCGGGTTATTCT CGGATGTAGCCAAGTGGATC 50.0 55.0 58.3 58.1 1679 bp 1.0 77.0
rpl2_p2 CGCTTCAACCGGGTTATTCT TGGATCAAGGCAGTGGATTG 50.0 50.0 58.3 57.9 1665 bp 1.0 77.0
rpl2_p3 CGCTTCAACCGGGTTATTCT GGATCAAGGCAGTGGATTGT 50.0 50.0 58.3 57.9 1664 bp 1.0 77.0
rpl2_p4 CGCTTCAACCGGGTTATTCT GTGGATCAAGGCAGTGGATT 50.0 50.0 58.3 57.9 1666 bp 1.0 77.0
rpl2_p5 ATCATTACGCTTCAACCGGG CGGATGTAGCCAAGTGGATC 50.0 55.0 58.3 58.1 1686 bp 1.0 76.5