Hotspot

ndhD

SSC · 118311-119816 · 1506 bp

Back to Hippeastrum

Pi 0.00188
MarkerSeek score 36.70
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1506
variable_sites
9
pi
0.00188
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 AGACTTTGCTTTCTGTCTCA TGGCCAAAACGTATCTTGTCT 40.0 42.9 54.5 57.9 1564 bp 1.0 43.6
ndhD_p2 AAGACTTTGCTTTCTGTCTCA TGGCCAAAACGTATCTTGTCT 38.1 42.9 55.4 57.9 1565 bp 1.0 42.4
ndhD_p3 AGACTTTGCTTTCTGTCTCAT TGGCCAAAACGTATCTTGTCT 38.1 42.9 55.2 57.9 1564 bp 1.0 40.6
ndhD_p4 AGACTTTGCTTTCTGTCTCA TGGCCAAAACGTATCTTGTC 40.0 45.0 54.5 56.4 1564 bp 1.0 40.5
ndhD_p5 AGACTTTGCTTTCTGTCTCATT TGGCCAAAACGTATCTTGTCT 36.4 42.9 55.9 57.9 1564 bp 1.0 40.5