Hotspot

rpl20-rps12

LSC · 71247-71987 · 741 bp

Back to Hippeastrum

Pi 0.0027

MarkerSeek score 48.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 6
pi: 0.0027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTGTTGAGTAGTGGTTCGGG	AATATCACGAAATCCCCCGC	55.0	50.0	57.9	58.1	920-926 bp	1.0	79.1
rpl20-rps12_p2	CTGTTGAGTAGTGGTTCGGG	TCGGGGAACATGTACTAGGG	55.0	55.0	57.9	58.2	879-885 bp	1.0	78.3
rpl20-rps12_p3	TTTGTTCGACGTCTCCTAGC	AATATCACGAAATCCCCCGC	50.0	50.0	57.7	58.1	853-859 bp	1.0	77.0
rpl20-rps12_p4	TGTTCGACGTCTCCTAGCTA	AATATCACGAAATCCCCCGC	50.0	50.0	57.6	58.1	851-857 bp	1.0	76.7
rpl20-rps12_p5	TTTGTTCGACGTCTCCTAGC	TCGGGGAACATGTACTAGGG	50.0	55.0	57.7	58.2	812-818 bp	1.0	76.2