Hotspot

psaJ-rpl33

LSC · 69459-69946 · 488 bp

Back to Hippeastrum

Pi 0.00233

MarkerSeek score 51.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 5
pi: 0.00233
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	CCGGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	650-657 bp	1.0	80.5
psaJ-rpl33_p2	ATGGTTTGGGTCTTTAGCGG	CCGGCGATTCCTTATTGACA	50.0	50.0	58.2	58.1	655-662 bp	1.0	79.6
psaJ-rpl33_p3	TGGGTCTTTAGCGGGTCTAT	CCGGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	649-656 bp	1.0	79.6
psaJ-rpl33_p4	TTGGGTCTTTAGCGGGTCTA	ATATCTAGAAATGCCCGGCG	50.0	50.0	58.1	57.6	664-671 bp	1.0	77.8
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCGG	ATATCTAGAAATGCCCGGCG	50.0	50.0	58.2	57.6	669-676 bp	1.0	76.9